RNA interference for wheat functional gene analysis
نویسندگان
چکیده
منابع مشابه
RNA interference-based gene silencing as an efficient tool for functional genomics in hexaploid bread wheat.
Insertional mutagenesis and gene silencing are efficient tools for the determination of gene function. In contrast to gain- or loss-of-function approaches, RNA interference (RNAi)-induced gene silencing can possibly silence multigene families and homoeologous genes in polyploids. This is of great importance for functional studies in hexaploid wheat (Triticum aestivum), where most of the genes a...
متن کاملGenome Analysis RNA Interference-Based Gene Silencing as an Efficient Tool for Functional Genomics in Hexaploid Bread Wheat
Insertional mutagenesis and gene silencing are efficient tools for the determination of gene function. In contrast to gainor lossof-function approaches, RNA interference (RNAi)-induced gene silencing can possibly silence multigene families and homoeologous genes in polyploids. This is of great importance for functional studies in hexaploid wheat (Triticum aestivum), where most of the genes are ...
متن کاملRna Interference: Tool for Gene Silencing
Over the past two decades, dramatic advances in the role of RNA in normal health and disease have been greatly expanded. During development Small RNA molecules regulate eukaryotic gene expression and also during responses to stress including viral infection. RNA interference (RNAi) is a highly conserved process in eukaryotes that leads to post-transcriptional gene silencing through degradation ...
متن کاملFunctional genomic analysis of RNA interference in C. elegans.
RNA interference (RNAi) of target genes is triggered by double-stranded RNAs (dsRNAs) processed by conserved nucleases and accessory factors. To identify the genetic components required for RNAi, we performed a genome-wide screen using an engineered RNAi sensor strain of Caenorhabditis elegans. The RNAi screen identified 90 genes. These included Piwi/PAZ proteins, DEAH helicases, RNA binding/pr...
متن کاملA functional genomic analysis of cell morphology using RNA interference
BACKGROUND The diversity of metazoan cell shapes is influenced by the dynamic cytoskeletal network. With the advent of RNA-interference (RNAi) technology, it is now possible to screen systematically for genes controlling specific cell-biological processes, including those required to generate distinct morphologies. RESULTS We adapted existing RNAi technology in Drosophila cell culture for use...
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ژورنال
عنوان ژورنال: Transgenic Research
سال: 2007
ISSN: 0962-8819,1573-9368
DOI: 10.1007/s11248-007-9150-7